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redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

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GENTAUR Italy

23015 Milano, Italy

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0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

ELISA Starter Kit (Casein) pink-ONE
Catalog Number K0331001P
Size 1000well
Type ELISA Kit
Datasheet
 
Specificity
This Kit is used to quantitatively measure levels of proteins or specific antigens in serum or other biological samples by using with EzWay ELISA Core kits. Except antibody & standard, all other reagents for full ELISA test are supplied.
 
Description
  • There is no need to buy extra solutions separately.
  • Minimize the cost and time for preparation of full ELISA Kit.
  • All kit components are optimized for common ELISA test.
  • It is convenient to use together with Core kits.
  • Pink-color coded indicator : Convenient to track for reagent supply
 
Kit Contents
Items Quantity
ELISA Microtiter Wells Plate 10 plates
Coating Buffer (pH9.6) 125ml x 2
Blocking Solution (1% BSA/PBS) 125ml x 2
PBS Powder Pouch for 1L x 5
Tween-20 (50%) 1ml (50%) x 5
pink-ONE TMB Solution 100ml
Stop Solution (2M H2SO4) 60ml
 
Storage & Shelf life
1 year at 2-8°C
 
Buffer Preparation
  1. Coating Buffer: 50 mM Carbonate-Bicarbonate Buffer, pH 9.6. Resolve the coating material (antigen or antibody) in the coating buffer to make 1ug/ml (1-10ug/ml).
  2. Sample/Standard/Antibody Diluting Solution : Dilute Sample/Standard/Antibody in PBS (Phosphate Buffered Saline, pH7.4). Or use PBST (Washing solution) or Blocking solution instead of PBS to help prevent non-specific binding.
  3. Washing Solution: Add 50% 1ml Tween 20 to 1 Liter PBS and mix well.
 
Sandwich ELISA Protocol
  1. Coating
    1. Dispense 200ul (may vary from 50 to 200ul depending on user's need) of prepared Coating Solution to each well.
    2. Incubate 1 hour at 37°C. (or 1-3 hours at room temperature/overnight at 4°C)
  2. Washing (All washing method is the same)
    1. Remove the solution from each well and fill up the Washing Solution. Repeat 3-5 times. Complete removal of liquid in each step is essential to good performance.
    2. After the last wash, remove any remaining Washing Solution. Turn over the plate and blot carefully on paper towels.
  3. Blocking
    1. Add 250ul Blocking Solution to each well.
    2. Incubate 1 hour at room temperature or at 37°C.
  4. Washing
  5. React Sample/Standard (or Primary Antibody)
    1. Add 200ul diluted Sample/Standard (or Primary Antibody) to each well.
    2. Incubate 1 hour at room temperature or at 37°C.
  6. Washing
  7. Add HRP-conjugated Detection Antibody (or Secondary Antibody)
    1. Add 200ul diluted Detection Antibody to each well.
    2. Incubate 1 hour at room temperature or at 37°C.
  8. Washing
  9. Color Reaction and Reading
    1. Dispense 150ul Color Reaction Mixture into each well. (Or apply 50ul TMB Solution and 100ul Substrate Solution to each well. Mix well by vortexing or shaking slightly.)
    2. After sufficient color development (5-10 minutes at room temperature or at 37°C), add 50ul Stop Solution (2M H2SO4) to each well.
    3. Read plates in a microwell plate reader at wavelength setting of 450nm.