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Carboxyfluorescein Caspase Detection Kits ™
Apoptosis detection and in situ labeling of active caspase in live cells.
 
Key Benefits:
  • Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
  • Cell permeablity permits direct visualization of cytosolic apoptotic events.
  • Apoptotic cell population does not diminish over time.
  • Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
  • Works in diverse cell lines: human, rodent, Drosophila.
  • Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
  • Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
  • Works with fluorescence microscopy, 96-well fluorescence plate readers, or flow cytometry.
  • Yields both quantitative and qualitative results. Gives strong signal with little background noise.
  • Mark activity across the range of caspase proteins. Poly-caspase and caspase-specific assays to target caspases 1, 2, 3, 6, 8, 9, or 10 are available.
  • Applications - Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscope
Assay Principle
APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.
Kit contents
  • Lyophilized FAM labeled peptide inhibitor
  • 10X Wash Buffer
  • 10X Fixative
  • Propidium Iodide
The following kits are available:
Product
 
Catalog No.
 
Size
(No. of Tests)
Price (Eur)
FAM-VAD-FMK Poly caspase detection kit FAM100-1 25 €145
FAM100-2
 
100 €425
FAM-DEVD-FMK Caspase 3 detection kit
 
FAM200-1 25 €145
FAM200-2
 
100 €425
FAM-LETD-FMK Caspase 8 detection kit FAM300-1 25 €155
FAM300-2
 
100 €445
FAM-LEHD-FMK Caspase 9 detection kit FAM400-1 25 €145
FAM400-2
 
100 €425
FAM-VEID-FMK Caspase 6 detection kit FAM500-1 25 €195
FAM500-2
 
100 €495
FAM-YVAD-FMK Caspase 1 detection kit FAM600-1 25 €155
FAM600-2
 
100 €445
FAM-VDVAD-FMK Caspase 2 detection kit FAM700-1 25 €195
FAM700-2
 
100 €495
FAM-AEVD-FMK Caspase 10 detection kit FAM800-1 25 €195
FAM800-2
 
100 €495

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