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redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

Block Buffers
Improves Assays:
  • Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein.
  • Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal.
  • Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency.
  • Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.
Blockers Available:
EBB100 General-Purpose Blocker with BSA-Based Blockers
EBB200 Non-Mammalian-Based Blocker
EBB300 Synthetic Blocker with Tween
Directions:
Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).
EBB100 General-Purpose Blocker with BSA-Based Blockers
General-Purpose Blocker contains a mammalian protein blocking agent (based on BSA) suitable for most monoclonal and polyclonal antibody capture ELISA formats, as well as peptide and protein antigen-down ELISA formats. This unique block buffer contains proprietary protein stabilizers that provide a long-term stabilizing environment for dried antigen or antibody coat proteins, while minimizing nonspecific binding interactions during the assay process.
Catalog Number Size #Plates at 300uL/well
(28.8mL/plate)
 
#Plates at 400uL/well
(38.4mL/plate)
Price
EBB100-1 100 3 2 €45.00
EBB100-2 500
 
16 12 €120.00
EBB100-3 1,000 33 25 €199.00
EBB100-4 10,000 330 250 €1900.00
EBB200 Nonmammalian-Based Blocker
Cell Technology's Nonmammalian-Based Blocker contains a proprietary non-mammalian formulation based on fish proteins, plus molecular stabilizers that provide a high degree of blocking efficiency. EBB200 was designed for antigen-down ELISA formats, and for sandwich assays with high background problems. This formulation is a heterogeneous mixture of small molecules capable of blocking both the nonspecific binding sites of the absorbed protein, as well as the unoccupied regions of the polystyrene plate that are not sterically accessible to larger traditional mammalian blocking reagents, thereby reducing background noise. The small size of these unique blockers results in minimal steric hindrance to key epitope regions of target coated proteins, thus enhancing specific signal generation.

EBB200 reduces the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate blocking proteins. It is particularly useful when working with human, swine, and bovine serum samples, as there is little interaction with the blocking molecules resulting in lower backgrounds.

Catalog Number Size #Plates at 300uL/well
(28.8mL/plate)
 
#Plates at 400uL/well
(38.4mL/plate)
Price
EBB200-1 100 3 2 €45.00
EBB200-2 500
 
16 12 €140.00
EBB200-3 1,000 33 25 €270.00
EBB200-4 10,000 330 250 €2500.00
EBB300 Synthetic Blocker
Cell Technology's Synthetic Blocker is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because Synthetic Blocker contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
Catalog Number Size #Plates at 300uL/well
(28.8mL/plate)
 
#Plates at 400uL/well
(38.4mL/plate)
Price
EBB300-1 100 3 2 €45.00
EBB300-2 500
 
16 12 €140.00
EBB300-3 1,000 33 25 €270.00
EBB300-4 10,000 330 250 €2300.00