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redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

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SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

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Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

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GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

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23015 Milano, Italy

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OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

Apo ssDNA™
An antibody assay to detect DNA Damage (Single Stranded) in apoptotic cells

Key Benefits:
  • Much more Reliable as compared to TUNEL assays - No False positive signals
  • Readout - Flow cytometry, 96 well plate reader, Fluorescence microscope .
  • Yields both quantitative and qualitative results. Gives a strong positive signal. 
  • Ease Of Use: Results in less than 60 minutes
  • No need to run DNA  ladder assays to detect DNA damage in cells
Introduction:

A widely used cytochemical technique for evaluation of DNA damage associated with apoptosis is the terminal deoxynucleotidyl transferase-mediated in situ end labeling or TUNEL assay. However the TUNEL assay has its drawbacks in that false positive staining makes the assay unreliable as a marker for apoptosis (see figure 1) 1-5. A more universal and specific marker for apoptosis is the morphological changes in nuclei that reflect chromatin condensation in to compact masses 6-7.

Further biochemical and cytochemical studies have demonstrated the increased susceptibility of apoptotic DNA to thermal denaturation. Analysis of nuclei by scanning calorimetry to detect thermal induced DNA denaturation and analysis of DNA fragmentation by electrophoresis have shown that intact apoptotic DNA is susceptible to denaturation at lower temperatures then that of non-apoptotic cells 8.
Assay Principle:
Cell Technology introduces Apo ssDNA, an antibody based assay to detect DNA damage (single stranded DNA: ssDNA) in Apoptotic Cells.

The assay utilizes an antibody generated against ssDNA. This antibody recognizes large stretches of heat-denatured ssDNA only in apoptotic cells 9-12

Kit contents and Long Term storage:
1. Mouse anti single stranded DNA…………………  Part  1003              -70°C aliquot

2. Anti mouse IgM FITC labeled……………………    Part  2005              -20°C

3. Block Buffer…………………………..…………………………… Part  3033              4-8°C

4. 10 X Wash Buffer……………………………………………    Part  3026              4-8°C

The following kits are available:
Catalog
 
Size Price

APODNA-1 

25 Tests €225
APODNA-2  100 Tests €745

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