Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] Antibody Mediated Cytotoxicity Home

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

ACT 1™
Assay for CytoToxicity
A non-radioactive cytotoxicity assay for flow cytometry
Key Benefits:
  • No radioactive materials required
  • Applications - Works with a flow cytometer or fluorescence microscope
  • Detects cytolytic activity at a cellular level
  • Works with multiple types of mammalian cell lines
Technology:
The most commonly used method to measure CMC/ADCC is a radioactive chrominum-51 (51Cr) release assays (2). There are several disadvantages with this assay: it is expensive, difficult to load certain cell types, expensive to dispose of due to strict environmental regulations, and has high background from spontaneous release of 51Cr. With the use of flow cytometry, it is now possible to eliminate the need for radioactive material and increase the ability to quantify cytolytic activity on a single cell bases. Various groups have demonstrated that measuring CMC/ADCC activity by flow cytometry has a strong (95%) correlation with the traditional 51Cr release assay (3,4,5,6).
Assay Principle :
A cell tracking dye CFSE ( 7,8,9) is utilized to label the target cell population. After the assay has run its experimental protocol, 7AAD (live/dead) (10,11) is added to measure cell death. 7AAD only enters membrane-compromised cells and binds to DNA.

Flow cytometry is utilized to gate on the target cells and measure 7AAD negative vs 7AAD postitve cells. % cytotoxicity is calculated by the following equation (see experimental example below):

7AAD positive (upper right quadrant)=R1/ 7AAD Postitve (upper right quadrant)= R1 + 7AAD negative (lower right quadrant)=R2 x100

Kit contents:
  • 4 vials of CFSE membrane stain, part no 4002.
  • 3 vials of 7AAD Live/Dead stain, part no 4003.
The following kit is available:
Product
 
Catalog No.
 
Size
(No. of Tests)
Price
ACT 1
Assay for CytoToxicity

 
ACT100-2 100 €275